Journal article
Parallel analysis of multiple human memory CD4 T-cell subsets within antigen-specific responses using cell proliferation dyes
L Cook, J Zaunders, N Seddiki, D van Bockel, AD Kelleher, CML Munier
Immunology and Cell Biology | WILEY | Published : 2023
DOI: 10.1111/imcb.12606
Abstract
Activation induced marker (AIM) assays are being used increasingly to measure antigen-specific T-cell responses, but this activation can alter cell lineage defining phenotypic markers. We aimed to extend the utility of AIM assays to enable pre-activation defined cell populations to be tracked and quantified within T-cell memory responses. We sorted three ex vivo CD4+ T-cell populations prior to any activation using well defined ex vivo lineage surface marker combinations. These populations were memory non-Tregs, CD39+ Tregs and CD39neg Tregs, although any three memory CD4+ T-cell populations able to be isolated by cell surface markers could potentially be tracked. These cells were labeled wi..
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Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
The authors thank all the healthy donors for their cooperation in providing blood samples and Ms Michelle Bailey and Dr Yin Xu for assistance with flow cytometric isolation of T cell populations. This work was funded by the following sources: Australian Government Department of Health and Aging; the NHMRC via a Program (510448) grant, a Practitioner Fellowship (ADK); and an Australian Postgraduate Award and a UNSW Research Excellence Scholarship (LC). NS, JZ and ADK, in conjunction with St Vincent's Hospital, Sydney, Australia, hold a patent for the CD25+CD134+CD39+ assay (PCT/AU2008/001407). Open access publishing facilitated by The University of Melbourne, as part of the Wiley - The University of Melbourne agreement via the Council of Australian University Librarians.